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endothelial cell base medium  (PromoCell)


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    Structured Review

    PromoCell endothelial cell base medium
    Endothelial Cell Base Medium, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 224 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+base+medium/Endothelial+Cell+Growth+Medium/pm40639610-47-23-28
    Average 96 stars, based on 224 article reviews
    endothelial cell base medium - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Isolation:

    Article Title: Recombinant pseudocowpoxvirus
    Article Snippet: .. Briefly, after isolation from PBMCs obtained from healthy donors either by positive CD14+ selection or by negative selection using monocyte isolation kit II (Miltenyi, Biotec), 4×105 monocytes were cultured on 48 well plates in 500 μl Macrophage Base Medium DXF (Promocell) supplemented with 50 ng/ml M-CSF (Miltenyi Biotec). ..

    Selection:

    Article Title: Recombinant pseudocowpoxvirus
    Article Snippet: .. Briefly, after isolation from PBMCs obtained from healthy donors either by positive CD14+ selection or by negative selection using monocyte isolation kit II (Miltenyi, Biotec), 4×105 monocytes were cultured on 48 well plates in 500 μl Macrophage Base Medium DXF (Promocell) supplemented with 50 ng/ml M-CSF (Miltenyi Biotec). ..

    Cell Culture:

    Article Title: Recombinant pseudocowpoxvirus
    Article Snippet: .. Briefly, after isolation from PBMCs obtained from healthy donors either by positive CD14+ selection or by negative selection using monocyte isolation kit II (Miltenyi, Biotec), 4×105 monocytes were cultured on 48 well plates in 500 μl Macrophage Base Medium DXF (Promocell) supplemented with 50 ng/ml M-CSF (Miltenyi Biotec). ..

    Article Title: NF-κB-mediated cytokine secretion and glutamate metabolic reprogramming converge in breast cancer brain tropism.
    Article Snippet: E0771 were grown in RPMI medium (SIAL-RPMI-A), supplemented with 100 IU/ml penicillin-streptomycin (P4458-100; Sigma-Aldrich), 10 % fetal bovine serum (FBS-12A; Capricorn), and 1 % HEPES (H0887-100 ml Sigma-Aldrich). .. HCMEC/D3 were purchased from Merck KGaA (Darmstadt, Germany) and seeded on plastic cell culture plates coated with collagen (C-3867-1vl; Sigma-Aldrich) and grown in Endothelial Cell Base Medium, (PC-22210; Promo Cell), supplemented with 100 IU/ml penicillin-streptomycin (P4458-100; Sigma-Aldrich/Merck) and SupplementPack Endothelial Cell Growth Medium (C-39210; Promo Cell, Heidelberg, Germany). ..

    Article Title: Methods of mediating macrophage phenotypes
    Article Snippet: Peripheral blood monocytes were then acquired from the same donors by following Ficoll separation techniques (Stem Cell Technologies, Vancouver, Canada). .. The monocytes were subsequently cultured using peripheral blood cell macrophage control media (Macrophage Base Medium DXF; Promocell, Heidelberg, Germany) or control media with 10% PPP, 25% PPP, 10% APS, or 25% APS. ..

    Article Title: Combination of laser-generated silicon NPs and electrospun-nanofibres for the development of new-generation bioactive wound dressings.
    Article Snippet: In vitro cell cultures Murine myoblasts C2C12 (ATCC) were cultured according to a previously published protocol.15 Themedium consisted of DMEM GlutaMAX (Gibco) supplemented with 10% heat inactivated Foetal Bovine Serum (Euroclone), penicillin (100 IU mL−1, Gibco) and streptomycin (100 mg mL−1, Gibco). .. Healthy human keratinocytes HaCat (ATCC) were cultured in PromoCell's Keratinocyte Base Medium 2, supplemented with penicillin (100 IUmL−1, Gibco) and streptomycin (100 mg mL−1, Gibco). .. The cells and media were placed in T-75 asks (Sarstedt) at 37 °C and 5% CO2.

    Article Title: Combination of laser-generated silicon NPs and electrospun-nanofibres for the development of new-generation bioactive wound dressings
    Article Snippet: The medium consisted of DMEM GlutaMAX (Gibco) supplemented with 10% heat inactivated Foetal Bovine Serum (Euroclone), penicillin (100 IU mL −1 , Gibco) and streptomycin (100 mg mL −1 , Gibco). .. Healthy human keratinocytes HaCat (ATCC) were cultured in PromoCell's Keratinocyte Base Medium 2, supplemented with penicillin (100 IU mL −1 , Gibco) and streptomycin (100 mg mL −1 , Gibco). .. The cells and media were placed in T-75 flasks (Sarstedt) at 37 °C and 5% CO 2 .

    Control:

    Article Title: Methods of mediating macrophage phenotypes
    Article Snippet: Peripheral blood monocytes were then acquired from the same donors by following Ficoll separation techniques (Stem Cell Technologies, Vancouver, Canada). .. The monocytes were subsequently cultured using peripheral blood cell macrophage control media (Macrophage Base Medium DXF; Promocell, Heidelberg, Germany) or control media with 10% PPP, 25% PPP, 10% APS, or 25% APS. ..

    other:

    Article Title: Single-nucleus and spatial landscape of the sub-ventricular zone in human glioblastoma
    Article Snippet: Macrophage Base Medium XF , PromoCell , Cat. No. C-28057.



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    ATCC prostate epithelial cell base medium
    Cell viability, cell morphology, and nuclear morphology of butein against prostate cancer cell lines. ( A ) Chemical structure of butein. Formula: C 15 H 12 O 5 ; MW: 272.3. PC-3, DU145, and HPrEC cells were treated with butein at various concentrations (0, 1.25, 2.5, 5, 10, 15, 20, and 30 μM) and then cultured for 48 h. Viable cells were subjected to ( B ) trypan blue exclusion assay, and ( C ) MTT assay. ( D ) Cell morphology of PC-3 and DU145 cells was examined by phase contrast microscopy. ( E ) Nuclear morphology of PC-3 and DU145 cells was examined by fluorescence microscopy after DAPI (2 μg/mL) staining. All data are expressed as mean ± standard error of the mean of three independent experiments. *, p < 0.05 was compared to respective controls. HPrEC: human prostate <t>epithelial</t> cell line; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; and Conc., concentration.
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    STEMCELL Technologies Inc immunocult nk cell base medium
    <t>NK</t> <t>cell</t> characteristics after different serum‐free culture conditions. (A) Workflow for static and shaking NK‐92 cultures, and purification of EVs. (B) Viability of NK‐92 cells after 48 h of culture in serum‐free media under static or shaking conditions supplemented with IL‐15 was assessed by propidium iodide by flow cytometry. Control samples were NK‐92 cells grown in Advanced RPMI medium with 20% FBS. Data are presented as the mean ± SD of four separate experiments. Statistical analysis performed using the Friedman one‐way ANOVA test indicated no statistical difference between the different culture conditions. (C) Expression levels of CD56 on NK‐92 cells after 48 h of culture in serum‐free media under static or shaking conditions. One representative experiment of three.
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    Image Search Results


    Cell viability, cell morphology, and nuclear morphology of butein against prostate cancer cell lines. ( A ) Chemical structure of butein. Formula: C 15 H 12 O 5 ; MW: 272.3. PC-3, DU145, and HPrEC cells were treated with butein at various concentrations (0, 1.25, 2.5, 5, 10, 15, 20, and 30 μM) and then cultured for 48 h. Viable cells were subjected to ( B ) trypan blue exclusion assay, and ( C ) MTT assay. ( D ) Cell morphology of PC-3 and DU145 cells was examined by phase contrast microscopy. ( E ) Nuclear morphology of PC-3 and DU145 cells was examined by fluorescence microscopy after DAPI (2 μg/mL) staining. All data are expressed as mean ± standard error of the mean of three independent experiments. *, p < 0.05 was compared to respective controls. HPrEC: human prostate epithelial cell line; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; and Conc., concentration.

    Journal: Life

    Article Title: Effect of Butein, a Plant Polyphenol, on Apoptosis and Necroptosis of Prostate Cancer Cells in 2D and 3D Cultures

    doi: 10.3390/life15060836

    Figure Lengend Snippet: Cell viability, cell morphology, and nuclear morphology of butein against prostate cancer cell lines. ( A ) Chemical structure of butein. Formula: C 15 H 12 O 5 ; MW: 272.3. PC-3, DU145, and HPrEC cells were treated with butein at various concentrations (0, 1.25, 2.5, 5, 10, 15, 20, and 30 μM) and then cultured for 48 h. Viable cells were subjected to ( B ) trypan blue exclusion assay, and ( C ) MTT assay. ( D ) Cell morphology of PC-3 and DU145 cells was examined by phase contrast microscopy. ( E ) Nuclear morphology of PC-3 and DU145 cells was examined by fluorescence microscopy after DAPI (2 μg/mL) staining. All data are expressed as mean ± standard error of the mean of three independent experiments. *, p < 0.05 was compared to respective controls. HPrEC: human prostate epithelial cell line; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; and Conc., concentration.

    Article Snippet: HPrEC cells were cultured in Prostate Epithelial Cell Base Medium (ATCC, PCS-440-030) supplemented with (ATCC, PCS-440-040).

    Techniques: Cell Culture, Trypan Blue Exclusion Assay, MTT Assay, Microscopy, Fluorescence, Staining, Concentration Assay

    NK cell characteristics after different serum‐free culture conditions. (A) Workflow for static and shaking NK‐92 cultures, and purification of EVs. (B) Viability of NK‐92 cells after 48 h of culture in serum‐free media under static or shaking conditions supplemented with IL‐15 was assessed by propidium iodide by flow cytometry. Control samples were NK‐92 cells grown in Advanced RPMI medium with 20% FBS. Data are presented as the mean ± SD of four separate experiments. Statistical analysis performed using the Friedman one‐way ANOVA test indicated no statistical difference between the different culture conditions. (C) Expression levels of CD56 on NK‐92 cells after 48 h of culture in serum‐free media under static or shaking conditions. One representative experiment of three.

    Journal: Journal of Extracellular Biology

    Article Title: Evaluating the Influence of Different Serum‐Free Culture Conditions on the Production and Function of Natural Killer Cell‐Derived Extracellular Vesicles

    doi: 10.1002/jex2.70049

    Figure Lengend Snippet: NK cell characteristics after different serum‐free culture conditions. (A) Workflow for static and shaking NK‐92 cultures, and purification of EVs. (B) Viability of NK‐92 cells after 48 h of culture in serum‐free media under static or shaking conditions supplemented with IL‐15 was assessed by propidium iodide by flow cytometry. Control samples were NK‐92 cells grown in Advanced RPMI medium with 20% FBS. Data are presented as the mean ± SD of four separate experiments. Statistical analysis performed using the Friedman one‐way ANOVA test indicated no statistical difference between the different culture conditions. (C) Expression levels of CD56 on NK‐92 cells after 48 h of culture in serum‐free media under static or shaking conditions. One representative experiment of three.

    Article Snippet: NK‐92 cells (5 × 10 7 cells) were resuspended in 65 mL of serum‐free media with 10 ng/mL of recombinant human IL‐15 (R&D Systems) and cultured for 48 h. Six different serum‐free media optimized for culturing of NK cells were tested: CellGenix GMP SCGM (xeno‐free, Sartorius [abbreviated GMP SCGM]), CTS NK‐Xpander Medium (xeno‐free, ThermoFisher [abbreviated CTS]), Immunocult NK Cell Base Medium (xeno‐free, StemCell Technologies, abbreviated Immunocult), ExCellerate Human NK Cell Expansion Media, Xeno‐Free (R&D Systems, abbreviated ExCellerate XF), and ExCellerate Human NK Cell Expansion Media, Animal Component Free (R&D Systems, abbreviated ExCellerate ACF).

    Techniques: Purification, Flow Cytometry, Control, Expressing

    TEM images of purified EVs derived from different serum‐free NK cell culture media. (A) TEM images of EVs obtained from static culture conditions. (B) TEM images of EVs obtained from shaking culture conditions. Scale bar 200 nm. Images are representative of 8–10 images from each sample.

    Journal: Journal of Extracellular Biology

    Article Title: Evaluating the Influence of Different Serum‐Free Culture Conditions on the Production and Function of Natural Killer Cell‐Derived Extracellular Vesicles

    doi: 10.1002/jex2.70049

    Figure Lengend Snippet: TEM images of purified EVs derived from different serum‐free NK cell culture media. (A) TEM images of EVs obtained from static culture conditions. (B) TEM images of EVs obtained from shaking culture conditions. Scale bar 200 nm. Images are representative of 8–10 images from each sample.

    Article Snippet: NK‐92 cells (5 × 10 7 cells) were resuspended in 65 mL of serum‐free media with 10 ng/mL of recombinant human IL‐15 (R&D Systems) and cultured for 48 h. Six different serum‐free media optimized for culturing of NK cells were tested: CellGenix GMP SCGM (xeno‐free, Sartorius [abbreviated GMP SCGM]), CTS NK‐Xpander Medium (xeno‐free, ThermoFisher [abbreviated CTS]), Immunocult NK Cell Base Medium (xeno‐free, StemCell Technologies, abbreviated Immunocult), ExCellerate Human NK Cell Expansion Media, Xeno‐Free (R&D Systems, abbreviated ExCellerate XF), and ExCellerate Human NK Cell Expansion Media, Animal Component Free (R&D Systems, abbreviated ExCellerate ACF).

    Techniques: Purification, Derivative Assay, Cell Culture